diff --git a/cura/CuraApplication.py b/cura/CuraApplication.py
index c133657a1f..08861af7c4 100755
--- a/cura/CuraApplication.py
+++ b/cura/CuraApplication.py
@@ -320,7 +320,7 @@ class CuraApplication(QtApplication):
         super().initialize()
 
         self._preferences.addPreference("cura/single_instance", False)
-        self._use_single_instance = self._preferences.getValue("cura/single_instance")
+        self._use_single_instance = self._preferences.getValue("cura/single_instance") or self._cli_args.single_instance
 
         self.__sendCommandToSingleInstance()
         self._initializeSettingDefinitions()