diff --git a/resources/definitions/builder_premium_large.def.json b/resources/definitions/builder_premium_large.def.json
index 3ceae8d63f..feb6082c55 100644
--- a/resources/definitions/builder_premium_large.def.json
+++ b/resources/definitions/builder_premium_large.def.json
@@ -55,7 +55,7 @@
         "prime_tower_wipe_enabled": { "default_value": false },
         "prime_tower_min_volume": { "default_value": 50 },
 
-        "prime_blob_enable": { "enabled": true },
+        "prime_blob_enable": { "enabled": "resolveOrValue('print_sequence') != 'one_at_a_time'" },
 
         "acceleration_enabled": { "value": "True" },
         "acceleration_layer_0": { "value": "acceleration_topbottom" },
diff --git a/resources/definitions/builder_premium_medium.def.json b/resources/definitions/builder_premium_medium.def.json
index 5f608ba2a8..cea28b4bea 100644
--- a/resources/definitions/builder_premium_medium.def.json
+++ b/resources/definitions/builder_premium_medium.def.json
@@ -55,7 +55,7 @@
         "prime_tower_wipe_enabled": { "default_value": false },
         "prime_tower_min_volume": { "default_value": 50 },
 
-        "prime_blob_enable": { "enabled": true },
+        "prime_blob_enable": { "enabled": "resolveOrValue('print_sequence') != 'one_at_a_time'" },
 
         "acceleration_enabled": { "value": "True" },
         "acceleration_layer_0": { "value": "acceleration_topbottom" },
diff --git a/resources/definitions/builder_premium_small.def.json b/resources/definitions/builder_premium_small.def.json
index a19773ec05..688a62d02c 100644
--- a/resources/definitions/builder_premium_small.def.json
+++ b/resources/definitions/builder_premium_small.def.json
@@ -54,7 +54,7 @@
         "prime_tower_wipe_enabled": { "default_value": false },
         "prime_tower_min_volume": { "default_value": 50 },
 
-        "prime_blob_enable": { "enabled": true },
+        "prime_blob_enable": { "enabled": "resolveOrValue('print_sequence') != 'one_at_a_time'" },
 
         "acceleration_enabled": { "value": "True" },
         "acceleration_layer_0": { "value": "acceleration_topbottom" },
diff --git a/resources/definitions/fdmprinter.def.json b/resources/definitions/fdmprinter.def.json
index ebbb6a5a3c..0c05fd45b8 100644
--- a/resources/definitions/fdmprinter.def.json
+++ b/resources/definitions/fdmprinter.def.json
@@ -4622,7 +4622,7 @@
                     "label": "Enable Prime Blob",
                     "description": "Whether to prime the filament with a blob before printing. Turning this setting on will ensure that the extruder will have material ready at the nozzle before printing. Printing Brim or Skirt can act like priming too, in which case turning this setting off saves some time.",
                     "type": "bool",
-                    "resolve": "any(extruderValues('prime_blob_enable'))",
+                    "resolve": "any(extruderValues('prime_blob_enable')) and resolveOrValue('print_sequence') != 'one_at_a_time'",
                     "default_value": false,
                     "settable_per_mesh": false,
                     "settable_per_extruder": true,
diff --git a/resources/definitions/ultimaker3.def.json b/resources/definitions/ultimaker3.def.json
index d2cd0376d3..1ad2579295 100644
--- a/resources/definitions/ultimaker3.def.json
+++ b/resources/definitions/ultimaker3.def.json
@@ -79,7 +79,7 @@
         "prime_tower_position_x": { "value": "machine_depth - max(extruderValue(adhesion_extruder_nr, 'brim_width') * extruderValue(adhesion_extruder_nr, 'initial_layer_line_width_factor') / 100 if adhesion_type == 'brim' else (extruderValue(adhesion_extruder_nr, 'raft_margin') if adhesion_type == 'raft' else (extruderValue(adhesion_extruder_nr, 'skirt_gap') if adhesion_type == 'skirt' else 0)), max(extruderValues('travel_avoid_distance'))) - max(extruderValues('support_offset')) - sum(extruderValues('skirt_brim_line_width')) - 30" },
         "prime_tower_wipe_enabled": { "default_value": false },
 
-        "prime_blob_enable": { "enabled": true, "default_value": true },
+        "prime_blob_enable": { "enabled": "resolveOrValue('print_sequence') != 'one_at_a_time'", "default_value": true },
 
         "acceleration_enabled": { "value": "True" },
         "acceleration_layer_0": { "value": "acceleration_topbottom" },
diff --git a/resources/definitions/ultimaker_s5.def.json b/resources/definitions/ultimaker_s5.def.json
index 0ebd956aa1..0fb94e8f55 100644
--- a/resources/definitions/ultimaker_s5.def.json
+++ b/resources/definitions/ultimaker_s5.def.json
@@ -69,7 +69,7 @@
         "machine_end_gcode": { "default_value": "" },
         "prime_tower_position_x": { "default_value": 345 },
         "prime_tower_position_y": { "default_value": 222.5 },
-        "prime_blob_enable": { "enabled": true, "default_value": false },
+        "prime_blob_enable": { "enabled": "resolveOrValue('print_sequence') != 'one_at_a_time'", "default_value": false },
 
         "speed_travel":
         {
diff --git a/resources/definitions/winbo_dragonl4.def.json b/resources/definitions/winbo_dragonl4.def.json
index bf52a785e9..7340f52739 100644
--- a/resources/definitions/winbo_dragonl4.def.json
+++ b/resources/definitions/winbo_dragonl4.def.json
@@ -41,7 +41,7 @@
         "machine_extruder_count": { "default_value": 1 },
         "machine_start_gcode": { "default_value": "G21\nG90\nM82\nM107\nM9998\nG28 X0 Y0\nG28 Z0\nG1 F6000 Z0.3\nG92 E0\nG1 F800 X585 E12\nG92 E0" },
         "machine_end_gcode": { "default_value": "M104 S0\nM140 S0\nG92 E2\nG1 E0 F200\nG28 X0 Y0\nM84 X Y E" },
-        "prime_blob_enable": { "enabled": true },
+        "prime_blob_enable": { "enabled": "resolveOrValue('print_sequence') != 'one_at_a_time'" },
         "acceleration_enabled": { "value": "True" },
         "acceleration_layer_0": { "value": "acceleration_topbottom" },
         "acceleration_prime_tower": { "value": "math.ceil(acceleration_print * 2000 / 4000)" },
diff --git a/resources/definitions/winbo_mini2.def.json b/resources/definitions/winbo_mini2.def.json
index f1c94ca07e..5b94b9a3fa 100644
--- a/resources/definitions/winbo_mini2.def.json
+++ b/resources/definitions/winbo_mini2.def.json
@@ -41,7 +41,7 @@
         "machine_extruder_count": { "default_value": 1 },
         "machine_start_gcode": { "default_value": "G21\nG90\nM82\nM107\nG28 X0 Y0\nG28 Z0\nG1 F1000 Z3\nG1 F4000 X0\nG1 F4000 Y0\nG1 F1000 Z0.2\nG92 E0\nG1 F1000 X30 E8\nG92 E0\nM117 Printing." },
         "machine_end_gcode": { "default_value": "M104 S0\nM140 S0\nG92 E2\nG1 E0 F200\nG28 X0 Y0\nM84 X Y E" },
-        "prime_blob_enable": { "enabled": true },
+        "prime_blob_enable": { "enabled": "resolveOrValue('print_sequence') != 'one_at_a_time'" },
         "acceleration_enabled": { "value": "True" },
         "acceleration_layer_0": { "value": "acceleration_topbottom" },
         "acceleration_prime_tower": { "value": "math.ceil(acceleration_print * 2000 / 4000)" },
diff --git a/resources/definitions/winbo_superhelper105.def.json b/resources/definitions/winbo_superhelper105.def.json
index ac78467a2a..0a10d3c7e9 100644
--- a/resources/definitions/winbo_superhelper105.def.json
+++ b/resources/definitions/winbo_superhelper105.def.json
@@ -41,7 +41,7 @@
         "machine_extruder_count": { "default_value": 1 },
         "machine_start_gcode": { "default_value": "G21\nG90\nM82\nM107\nG28 X0 Y0\nG28 Z0\nG1 F6000 Z0.3\nG92 E0\nG1 F1000 X30 E8\nG92 E0\nM117 Printing." },
         "machine_end_gcode": { "default_value": "M104 S0\nM140 S0\nG92 E2\nG1 E0 F200\nG28 X0 Y0\nM84 X Y E" },
-        "prime_blob_enable": { "enabled": true },
+        "prime_blob_enable": { "enabled": "resolveOrValue('print_sequence') != 'one_at_a_time'" },
         "acceleration_enabled": { "value": "True" },
         "acceleration_layer_0": { "value": "acceleration_topbottom" },
         "acceleration_prime_tower": { "value": "math.ceil(acceleration_print * 2000 / 4000)" },